Chemical isolation of a female urinary chemosignal which elicits ultrasonic vocalizations in male mice (mus musculus)

1 Chapter I: Introduction 2 Previous Attempts to Isolate Priming Pheromones 6 Puberty-accelerating Male Urinary Chemosignal 7 .. Pubert-delaying Female Urinary Chemosignal 12 Pregnancy-blocking Male Urinary Chemosignal 14 Estrus-synchronizing Male Urinary Chemosignal 17 Gonadotrophin-secretion Female Urinary Chemosignal 19 Similarities in the Nature of Priming Pheromones 21 Previous Attempts to Isolate· Releasirrg-Pneroniories--~--~~~----22~-----._-~Aggression-promoting Male Urinary Chemosignal 22 Aggression-reducing Female Urinary Chemosignal ~ Ultrasound-eliciting Female Urinary Chemosignal 28 Chapter II: Methods and Results Isolation of the Ultrasound Eliciting Female Urinary Chemosignal General Methods Animals Apparatus Procedure ---~--Social Experience Behavioral ~creening Stimulus Collection Chromatographic Procedure ill 31 31 32 32 33 . 33 34 34 Behavioral Testing Data Analysis Experiments 1-2: Sephadex G-I0 Separations Experiment 1 Experiment 2 Experiments 3-5; Sephadex G-15 Separartions Experiment 3 Experiment 4 Experiment 5 Chapter III: Discussion General Discussion Volatility Protein Characteristics Molecular Weight